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rabbit polyclonal antibody against il6st  (Bioss)


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    Bioss rabbit polyclonal antibody against il6st
    Upregulated <t>IL6ST</t> in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.
    Rabbit Polyclonal Antibody Against Il6st, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+il6st/pmc11391227-42-11-23?v=Bioss
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal antibody against il6st - by Bioz Stars, 2026-07
    94/100 stars

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    1) Product Images from "IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis"

    Article Title: IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2024.23353

    Upregulated IL6ST in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.
    Figure Legend Snippet: Upregulated IL6ST in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.

    Techniques Used: Expressing

    Knockdown of IL6ST could decrease viability and increase ferroptosis phenotype and related genes. (A-C) Effective knockdown of IL6ST in SW480 CRC cells is detected using RT-PCR. (B, C) Effective knockdown of IL6ST in SW480 CRC cells is detected using western blot. (D) Knockdown of IL6ST could significantly decrease the viability of SW480 CRC cells compared to nonmalignant human colonic epithelial cell lines NCM-460 and vector controls via CCK-8 assays ( P = .007 and P = .004, respectively). (E) IL6ST knockdown SW480 cells exhibit shrunken and damaged mitochondria (3200×). (F) Knockdown of IL6ST up-regulated the levels of iron in SW480 cells compared to the control group ( P = .007). (G) Knockdown of IL6ST increased the levels of ROS in SW480 cells compared to the control group ( P = .005). (H-L) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at the RNA levels, while FTH1 and GPX4 are downregulated in IL6ST knockdown SW480 cells ( P < .01). (M-R) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at protein levels in IL6ST knockdown SW480 cells, while FTH1 and GPX4 are upregulated in IL6ST overexpression SW620 cells ( P < .01).
    Figure Legend Snippet: Knockdown of IL6ST could decrease viability and increase ferroptosis phenotype and related genes. (A-C) Effective knockdown of IL6ST in SW480 CRC cells is detected using RT-PCR. (B, C) Effective knockdown of IL6ST in SW480 CRC cells is detected using western blot. (D) Knockdown of IL6ST could significantly decrease the viability of SW480 CRC cells compared to nonmalignant human colonic epithelial cell lines NCM-460 and vector controls via CCK-8 assays ( P = .007 and P = .004, respectively). (E) IL6ST knockdown SW480 cells exhibit shrunken and damaged mitochondria (3200×). (F) Knockdown of IL6ST up-regulated the levels of iron in SW480 cells compared to the control group ( P = .007). (G) Knockdown of IL6ST increased the levels of ROS in SW480 cells compared to the control group ( P = .005). (H-L) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at the RNA levels, while FTH1 and GPX4 are downregulated in IL6ST knockdown SW480 cells ( P < .01). (M-R) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at protein levels in IL6ST knockdown SW480 cells, while FTH1 and GPX4 are upregulated in IL6ST overexpression SW620 cells ( P < .01).

    Techniques Used: Knockdown, Reverse Transcription Polymerase Chain Reaction, Western Blot, Plasmid Preparation, CCK-8 Assay, Control, Over Expression

    IL6ST overexpression increased cell viability and decreased ferroptosis phenotype and related genes. (A,B) Effective overexpression of IL6ST in SW620 CRC cells is detected using western blot. (C) Overexpression of IL6ST increased the viability of SW620 CRC cells via CCK-8 assays compared to NCM-460 or vector control. (D) IL6ST overexpression protected mitochondrial integrity (3200×). (E) IL6ST overexpression decreased iron accumulation. (F) IL6ST overexpression down-regulated the production of ROS. (G-K) IL6ST up-regulated the expression of FTH1 and GPX4 and decreased expression of PTGS2, NOX1, and ACSL4 at RNA levels ( P < .01).
    Figure Legend Snippet: IL6ST overexpression increased cell viability and decreased ferroptosis phenotype and related genes. (A,B) Effective overexpression of IL6ST in SW620 CRC cells is detected using western blot. (C) Overexpression of IL6ST increased the viability of SW620 CRC cells via CCK-8 assays compared to NCM-460 or vector control. (D) IL6ST overexpression protected mitochondrial integrity (3200×). (E) IL6ST overexpression decreased iron accumulation. (F) IL6ST overexpression down-regulated the production of ROS. (G-K) IL6ST up-regulated the expression of FTH1 and GPX4 and decreased expression of PTGS2, NOX1, and ACSL4 at RNA levels ( P < .01).

    Techniques Used: Over Expression, Western Blot, CCK-8 Assay, Plasmid Preparation, Control, Expressing

    Correlation between IL6ST and immune cell subtype infiltration. (A) The expression level of IL6ST is positively correlated with the infiltration of T helper cells, macrophages, Tcm cells, Tgd cells, Mast cells, DC cells, Th1 cells, TFH cells, Th2 cells, neutrophils and T cells ( P < .05). (B) The enrichment score of T cells is significantly higher in IL6ST high-expressed group than in the low-expressed group.
    Figure Legend Snippet: Correlation between IL6ST and immune cell subtype infiltration. (A) The expression level of IL6ST is positively correlated with the infiltration of T helper cells, macrophages, Tcm cells, Tgd cells, Mast cells, DC cells, Th1 cells, TFH cells, Th2 cells, neutrophils and T cells ( P < .05). (B) The enrichment score of T cells is significantly higher in IL6ST high-expressed group than in the low-expressed group.

    Techniques Used: Expressing



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    rabbit polyclonal antibody against il6st  (Bioss)
    94
    Bioss rabbit polyclonal antibody against il6st
    Upregulated <t>IL6ST</t> in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.
    Rabbit Polyclonal Antibody Against Il6st, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+il6st/pmc11391227-42-11-23?v=Bioss
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal antibody against il6st - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Upregulated IL6ST in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.

    Journal: The Turkish Journal of Gastroenterology

    Article Title: IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis

    doi: 10.5152/tjg.2024.23353

    Figure Lengend Snippet: Upregulated IL6ST in CRC tissues and cells was associated with ferroptosis-related genes. (A) IL6ST expression is upregulated in CRC tissues compared with unpaired controls based on TCGA-COAD database ( P = .000). (B) IL6ST expression is upregulated in CRC tissues compared with paired adjacent controls based on TCGA-COAD database ( P = .000). (C) Higher expression of IL6ST in CRC tissues is found compared to adjacent controls via IHC assay ( P = .0001) (200×). (D) IL6ST is upregulated in SW480, HCT116, HT-29, and SW620 cells than nonmalignant human colonic epithelial cell lines NCM-460. (E) Demonstrated the specificity and sensitivity of IL6ST in predicting CRC. (F) The expression level of IL6ST has a significant association with FTH1, GPX4, NCOA4, PTGS2, SLC7A11, and TFRC expression levels.

    Article Snippet: For IHC analysis, 5 µm tissue slides were added with a rabbit polyclonal antibody against IL6ST (1:100, diluted with 2% sheep serum, bs-34036R; Bioss, Boston, MA, USA) and incubated at 4°C overnight, followed by blocking with 5% sheep serum for 1 hour at 25°C.

    Techniques: Expressing

    Knockdown of IL6ST could decrease viability and increase ferroptosis phenotype and related genes. (A-C) Effective knockdown of IL6ST in SW480 CRC cells is detected using RT-PCR. (B, C) Effective knockdown of IL6ST in SW480 CRC cells is detected using western blot. (D) Knockdown of IL6ST could significantly decrease the viability of SW480 CRC cells compared to nonmalignant human colonic epithelial cell lines NCM-460 and vector controls via CCK-8 assays ( P = .007 and P = .004, respectively). (E) IL6ST knockdown SW480 cells exhibit shrunken and damaged mitochondria (3200×). (F) Knockdown of IL6ST up-regulated the levels of iron in SW480 cells compared to the control group ( P = .007). (G) Knockdown of IL6ST increased the levels of ROS in SW480 cells compared to the control group ( P = .005). (H-L) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at the RNA levels, while FTH1 and GPX4 are downregulated in IL6ST knockdown SW480 cells ( P < .01). (M-R) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at protein levels in IL6ST knockdown SW480 cells, while FTH1 and GPX4 are upregulated in IL6ST overexpression SW620 cells ( P < .01).

    Journal: The Turkish Journal of Gastroenterology

    Article Title: IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis

    doi: 10.5152/tjg.2024.23353

    Figure Lengend Snippet: Knockdown of IL6ST could decrease viability and increase ferroptosis phenotype and related genes. (A-C) Effective knockdown of IL6ST in SW480 CRC cells is detected using RT-PCR. (B, C) Effective knockdown of IL6ST in SW480 CRC cells is detected using western blot. (D) Knockdown of IL6ST could significantly decrease the viability of SW480 CRC cells compared to nonmalignant human colonic epithelial cell lines NCM-460 and vector controls via CCK-8 assays ( P = .007 and P = .004, respectively). (E) IL6ST knockdown SW480 cells exhibit shrunken and damaged mitochondria (3200×). (F) Knockdown of IL6ST up-regulated the levels of iron in SW480 cells compared to the control group ( P = .007). (G) Knockdown of IL6ST increased the levels of ROS in SW480 cells compared to the control group ( P = .005). (H-L) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at the RNA levels, while FTH1 and GPX4 are downregulated in IL6ST knockdown SW480 cells ( P < .01). (M-R) The expressions of PTGS2, NOX1, and ACSL4 are upregulated at protein levels in IL6ST knockdown SW480 cells, while FTH1 and GPX4 are upregulated in IL6ST overexpression SW620 cells ( P < .01).

    Article Snippet: For IHC analysis, 5 µm tissue slides were added with a rabbit polyclonal antibody against IL6ST (1:100, diluted with 2% sheep serum, bs-34036R; Bioss, Boston, MA, USA) and incubated at 4°C overnight, followed by blocking with 5% sheep serum for 1 hour at 25°C.

    Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Western Blot, Plasmid Preparation, CCK-8 Assay, Control, Over Expression

    IL6ST overexpression increased cell viability and decreased ferroptosis phenotype and related genes. (A,B) Effective overexpression of IL6ST in SW620 CRC cells is detected using western blot. (C) Overexpression of IL6ST increased the viability of SW620 CRC cells via CCK-8 assays compared to NCM-460 or vector control. (D) IL6ST overexpression protected mitochondrial integrity (3200×). (E) IL6ST overexpression decreased iron accumulation. (F) IL6ST overexpression down-regulated the production of ROS. (G-K) IL6ST up-regulated the expression of FTH1 and GPX4 and decreased expression of PTGS2, NOX1, and ACSL4 at RNA levels ( P < .01).

    Journal: The Turkish Journal of Gastroenterology

    Article Title: IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis

    doi: 10.5152/tjg.2024.23353

    Figure Lengend Snippet: IL6ST overexpression increased cell viability and decreased ferroptosis phenotype and related genes. (A,B) Effective overexpression of IL6ST in SW620 CRC cells is detected using western blot. (C) Overexpression of IL6ST increased the viability of SW620 CRC cells via CCK-8 assays compared to NCM-460 or vector control. (D) IL6ST overexpression protected mitochondrial integrity (3200×). (E) IL6ST overexpression decreased iron accumulation. (F) IL6ST overexpression down-regulated the production of ROS. (G-K) IL6ST up-regulated the expression of FTH1 and GPX4 and decreased expression of PTGS2, NOX1, and ACSL4 at RNA levels ( P < .01).

    Article Snippet: For IHC analysis, 5 µm tissue slides were added with a rabbit polyclonal antibody against IL6ST (1:100, diluted with 2% sheep serum, bs-34036R; Bioss, Boston, MA, USA) and incubated at 4°C overnight, followed by blocking with 5% sheep serum for 1 hour at 25°C.

    Techniques: Over Expression, Western Blot, CCK-8 Assay, Plasmid Preparation, Control, Expressing

    Correlation between IL6ST and immune cell subtype infiltration. (A) The expression level of IL6ST is positively correlated with the infiltration of T helper cells, macrophages, Tcm cells, Tgd cells, Mast cells, DC cells, Th1 cells, TFH cells, Th2 cells, neutrophils and T cells ( P < .05). (B) The enrichment score of T cells is significantly higher in IL6ST high-expressed group than in the low-expressed group.

    Journal: The Turkish Journal of Gastroenterology

    Article Title: IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis

    doi: 10.5152/tjg.2024.23353

    Figure Lengend Snippet: Correlation between IL6ST and immune cell subtype infiltration. (A) The expression level of IL6ST is positively correlated with the infiltration of T helper cells, macrophages, Tcm cells, Tgd cells, Mast cells, DC cells, Th1 cells, TFH cells, Th2 cells, neutrophils and T cells ( P < .05). (B) The enrichment score of T cells is significantly higher in IL6ST high-expressed group than in the low-expressed group.

    Article Snippet: For IHC analysis, 5 µm tissue slides were added with a rabbit polyclonal antibody against IL6ST (1:100, diluted with 2% sheep serum, bs-34036R; Bioss, Boston, MA, USA) and incubated at 4°C overnight, followed by blocking with 5% sheep serum for 1 hour at 25°C.

    Techniques: Expressing